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Portrait of Dr. Raul Braylan
Raul Braylan, MD

Senior Investigator
Chief, Hematology Service


Laboratory Medicine


MD, University of Buenos Aires


Email: raul.braylan@nih.gov
Phone:301-594-9556

Portrait of Dr. Raul Braylan
Raul Braylan, MD

Senior Investigator
Chief, Hematology Service


Laboratory Medicine


MD, University of Buenos Aires


Email: raul.braylan@nih.gov
Phone:301-594-9556


Dr. Raul Braylan has been interested in the pathobiology of hematologic malignancies and related disorders for many years.


Dr. Braylan received his medical degree from Buenos Aires Medical School in Argentina, and completed internship and residency in pathology at Mt. Sinai Hospital in Chicago and Albert Einstein College of Medicine in the Bronx. He was then a Senior Clinical Trainee for Cancer Control in the Pathology Department of Sloan Kettering Institute in New York and a U.S. Public Health Service Trainee in Hematopathology, and Instructor in the Department of Pathology at the University of Chicago.

He was subsequently appointed senior staff fellow in the National Cancer Institute at NIH in 1973, where he served for four years.

He then joined the Department of Pathology at the University of Florida in Gainesville, Florida, where he was the Chief of Hematopathology, Director of the Hematology course for medical students and Director of the Hematopathology Fellowship Training Program. Following his tenure at the UF, Dr. Braylan helped organize and direct the Hematopathology service at CARIS Life Sciences in Phoenix, AZ, and finally joined the Department of Laboratory Medicine in the Clinical Center at NIH in 2011.

Research Interests

Dr. Braylan has been interested in the pathobiology of hematologic malignancies and related disorders for many years. He was one of the pioneers in the clinical use of flow cytometry as a tool for diagnosis, characterization and monitoring these malignancies, and actively participated in international efforts to establish standardization in data analysis and reporting of clinical flow cytometric results.

He has mainly studied lymphoid malignancies in the past and he now focuses his efforts on myeloid and plasma cell disorders.

Among many other professional affiliations, Dr. Braylan is a member of the American Society of Hematology and the American Society of Clinical Pathologists. He has been an editorial board member of Cytometry Part B: Clinical Cytometry, and an associate editor at Laboratory Investigation.

He has published books, book chapters and more than 150 peer-reviewed articles in scientific journals.

See his Intramural Research Program bio page.

Original Articles

Hodes A, Calvo KR, Dulau A, Maric I, Sun J, Braylan, R. The challenging task of enumerating blasts in the bone marrow. Semin Hematol. 2019 Jan;56(1):58-64

Dulau-Florea AE, Young NS, Maric I, Calvo KR, Dunbar CE, Townsley DM, Winkler T, Monreal M, Jiang C, Jordan EK, Braylan, RC. Bone Marrow as a Source of Cells for Paroxysmal Nocturnal Hemoglobinuria Detection. Am J Clin Pathol. 2018 Jul;150(3):273–282

Davis, B.H., Holden, J.T., Bene, M.D., Borowitz, M.J., Braylan, R.C., Cornfield, D., Gorczyca, W., Lee, R. Maiese, R, Orfao, A., Wells, D., Wood, B. L., Stetler-Stevenson, M. 2006 Bethesda International Consensus Recommendations on the Flow Cytometric Immunophenotypic Analysis of Hematolymphoid Neoplasia: Medical Indications. Cytometry B Clin Cytom. 2007 72B:S13.

Al-Quran SZ, Yang L, Magill JM, Braylan RC, Douglas-Nikitin VK. Assessment of bone marrow plasma cell infiltrates in multiple myeloma: the added value of CD138 immunohistochemistry. Hum Pathol. 2007;38(12):1779-87.

Lee RV, Braylan RC, Rimsza LM. CD58 expression decreases as nonmalignant B cells mature in bone marrow and is frequently overexpressed in adult and pediatric precursor B-cell acute lymphoblastic leukemia. Am J Clin Pathol. 2005;123(1):119-24.

Braylan RC. Impact of flow cytometry on the diagnosis and characterization of lymphomas, chronic lymphoproliferative disorders and plasma cell neoplasias. Cytometry A. 2004;58(1):57-61.

Cornfield DB, Mitchell DM, Almasri NM, Anderson JB, Ahrens KP, Dooley EO, Braylan RC. Follicular lymphoma can be distinguished from benign follicular hyperplasia by flow cytometry using simultaneous staining of cytoplasmic bcl-2 and cell surface CD20. Am J Clin Pathol. 2000;114(2):258-63.

Books and Book Chapters

Fleisher TA and Braylan RC. (2013) Basic Principles and Clinical Applications of Flow Cytometry. In Bethesda Handbook of Clinical Hematology, 3rd Edition (Rodgers GP and Young NS, eds.) Philadelphia, PA: Lippincott Williams & Wilkins.

Allan RW, Al-Quran SZ., Li Y, and Braylan RC. (2012) Lymphoproliferative disorders. In Laboratory Hematology Practice, 1st Edition (Kottke-Marchant K. and Davis BH, eds), Blackwell Publishing, Ltd., Oxford, UK.

Nguyen, D.T., Diamond, L.W., Braylan R.C.. Flow Cytometry in Hematopathology: A Visual Approach to Data Analysis and Interpretation. Humana Press, Inc., Totowa, NJ. 1st and 2nd editions (2002 and 2007).

Douglas, V.K. and Braylan R.C. (2003) Immunophenotypic Differential Diagnosis and Cell Cycle Analysis. In: Chronic Lymphocytic Leukemia: Molecular Genetics, Biology, Diagnosis and Management. (Faguet, GB., ed), Humana Press, Inc. Totowa, NJ.

Braylan R.C. and Anderson, J. (2000) Flow Cytometric Analysis of Hematologic Neoplasia. In: Methods in Molecular Medicine, vol. 55: Hematologic Malignancies: Methods and Techniques (Faguet, GB., ed), Humana Press, Inc., Totowa, NJ. 215-228.

Braylan R.C. (1992). Lymphomas. In: Flow Cytometry: Principles and Clinical Applications. (Bauer, KD., Duque, RE., Shankey, TV., eds), 203-234. Baltimore, MD: Williams and Wilkins.

Moscinski, L.C. and Braylan R.C. (1992). Interpretation of Modern Diagnostic Techniques in Lymphoma. In: Manual of Clinical Laboratory Immunology (4th Ed.) (Rose, NR., de Macario, EC., Fahey, J.L., Friedman, H., Penn, GM., eds). Washington, D.C.: American Society of Microbiology.

Braylan R.C., Benson, N.A. and Nourse, V.A.: Flow Cytometry: A new approach toward characterizing lymphomas. B and T cell tumors: Biological and Clinical Aspects. UCLA Symposia on Molecular and Cellular Biology, Volume XXIV, (Eds.) Elen Vitetta and C. Fred Fox, Academic Press, New York, NY, 1982 73-77.

Rappaport, H., and Braylan R. C. (1975). The Changing Concepts in the Classification of Malignant Neoplasms of the Hematopoietic System. In: International Academy of Pathology Monographs No. 16: The Reticuloendothelial System (Rebuck, J.W., Berard, C.W., and Abell, M.R. eds.), 1 19. Baltimore: Williams & Wilkins Co.


  • Invited speaker at American Society for Clinical Laboratory Science Spring Symposium, Phoenix, AZ, 2010
  • Invited speaker at UCLA Hematopathology/BM & Hematology Multidisciplinary Conference, Los Angeles, 2009
  • Invited speaker at ASCP Workshops for Laboratory Professionals,2008
  • Invited speaker at ASCP Workshops for Laboratory Professionals, 2007
  • Invited speaker at Bone Marrow Transplantation Units, Daopei and Xuan Wu Hospitals, Beijing, China, 2007
  • Invited speaker at Asan Medical Center, Seoul, South Korea, 2007
  • Invited speaker at Pathology Grand Rounds, Dept. of Pathology, U. of Illinois, 2007
  • Whisenant Endowed Chair for Experimental Pathology, University of Florida College of Medicine, 1985
  • Fellowship of the Research National Council, Institute of Hematologic Investigations, Buenos Aires, Argentina, 1968
  • Senior Clinical Traineeship for Cancer Control, Memorial Hospital, Sloan Kettering Institute, New York, NY, 1967
  • Professor Emeritus at the University of Florida College of Medicine in Gainesville, FL.

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This page last updated on 05/17/2021

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