Equipment, Technology & Tools - Resources

High-Throughput Molecular Assays

Description Point of Contact Link

The Department of Transfusion Medicine has the capability of developing molecular assays for infectious agents, cytokines, tumor antigens, etc. on a variety of platforms.

Dr. Harvey Klein

Human Polyomavirus (JCV) DNA in Leukoenephalopathy (PML) and Brain Infection Detection Using Quantitative Polymerase Chain Reaction (qPCR) Assay

Description Point of Contact Link

The human polyomavirus, JCV, is the etiologic agent of the demyelinating disease, progressive multifocal leukoencephelopathy (PML). Real time polymerase chain reaction, qPCR, uses primers and probes specific to a highly conserved region of the JC Virus genome that has a sensitivity of 10 copies of viral DNA per ml of clinical sample, i.e. CSF, plasma/serum with 100% specificity. In addition to clinical signs, MRI evidence of brain lesions, the detection of viral DNA in CSF allows the laboratory confirmatory diagnosis of PML.

Gene Major, Ph.D.


Human Polyomaviruses (BKV and JCV) Antibody Measurement Using ELISA-Based Assay

Description Point of Contact Link

The JCV or BKV polyomaviruses are widely distributed in the human population. Measurement of antibodies directed to these viral pathogens is done using the major virion capsid protein, VP1, as the antigen cloned and expressed in Baculovirus vectors. Enzyme linked immunoabsorbent technology, ELISA, assays are used to titrate serum/plasma samples in 4 fold dilutions in plates treated with viral protein. Antibody levels are determined as the reciprocal of the highest dilution that gives an optical density of a chromophore above preset controls run on the same plate.

Gene Major, Ph.D.


Microbial Detection and Identification Using Mass Spectroscopy and Other Techniques

Description Point of Contact Link

The Clinical Microbiology Laboratory in the Department of Laboratory Medicine has developed two main approaches for microbial detection and identification: 1) Genome-based direct assays targeting viruses (e.g. respiratory viruses, EBV, HSV, CMV), fastidious bacteria (e.g. Borrelia, Legionella), fungi (e.g. Pneumocystis) and parasites (e.g. Toxoplasma, microfilaria) and 2) Mass spectrometry-based methods for organisms grown in culture (bacteria, yeasts, mycobacteria from plates or positive blood cultures).

Karen M. Frank, M.D., Ph.D.

Mutation Analysis Associated with Primary Immune Deficiency Disorders

Description Point of Contact Link

The Immunology Service has developed a mutation analysis program for primary immunodeficiency focusing on disorders seen at the NIH as part of the NIAID initiative in primary immunodeficiency disorders. This currently involves conventional Sanger sequencing of more than 40 different genes associated with these disorders and is also examining an alternative platform for initial screening of mutations in these genes to focus the Sanger sequencing to a specific gene and site.

Julie Niemela


Pathology Services

Description Point of Contact Link

The NCI Laboratory of Pathology provides clinical review of pathologic specimens. In addition, the lab develops and validates new assays and tests emerging assays in conjunction with research studies.

John Carl Oberholtzer, MD, PhD

Phagocyte Function

Description Point of Contact Link

The Neutrophil Monitoring Laboratory (NML) provides both clinical and basic research support to investigators in NIAID. The NLM primary mission is to provide CLIA-certified studies of phagocytic cell function on samples isolated from patients with suspected host defense defects. Studies focus on samples from patients seen at the NIH Clinical Center but with special approval also from samples shipped by express mail. Assays include determinations of reactive oxygen intermediates, staphylocidal activity, neutrophil adherence, chemotaxis, release of granular proteins and alteration of surface marker expression, cytokine expression among others. Gene sequencing is provided on patients suspected of specific defects studies at NIAID.

Douglas Kuhns


Pneumocystis Pneumonia Diagnosis

Description Point of Contact Link

Improved methods for diagnosing Pneumocystis pneumonia, a potentially fatal pneumonia of immunosuppressed patients.

Joseph A. Kovacs, M.D.

Karen M. Frank, M.D., Ph.D.

Cardiovascular Imaging: Advanced Cardiovascular Imaging Laboratory – MRI and CT

Description Point of Contact Link

The National Heart, Lung and Blood Institute (NHLBI) has extensive experience with cardiovascular MRI and cardiovascular CT in the context of a bench to bedside translational research program. Expertise includes technical developments in image acquisition, image reconstruction, quantitative post-processing, and both pre-clinical and clinical validations. The program provides expert clinical diagnostic evaluations for a wide range of diagnostic questions.

Andrew Arai, M.D. - MRI

Marcus Chen, - CT

Cardiovascular Imaging: Non-Invasive Imaging of Coronary Artery Disease, Heart Failure and Atherosclerosis Imaging

Description Point of Contact Link

The Radiology and Imaging Sciences Department is investigating noninvasive imaging methods for quantifying diffuse myocardial fibrosis with cardiac magnetic resonance imaging (CMR) and multi-detector computed tomography (MDCT) in heart failure patients.

Elizabeth C. Jones, M.D., M.P.H., M.B.A

Interventional Oncology (Drugs and Devices)

Description Point of Contact Link

The Center for Interventional Oncology, a partnership between the Clinical Center Radiology and Imaging Sciences Department and the National Cancer Institute, develops and translates technologies related to minimally invasive image guided therapies to address clinical needs, including navigation tools for biopsy and ablations, image guided drugs + devices and fusion navigation of “Medical GPS” enabled needles and catheters for local and regional cancer therapies.

Bradford Wood, M.D.

Intraoperative MRI for Real-Time Imaging During Procedures

Description Point of Contact Link

One of Clinical Center, Department of Perioperative Medicine's operating suites incorporates a 1.5 T MRI. This MRI offers the opportunity for 'real-time' imaging during neurosurgical procedures. The device is currently being used for a variety of procedures, including the evaluation of convection-enhanced delivery of chemotherapeutics to more accurately follow the volume of distribution of the cytotoxic agents.

Andrew Mannes, M.D., M.E.

Molecular Imaging: NCI Molecular Imaging Program

Description Point of Contact Link

Develops and validates new agents used in the imaging and potentially the therapy of tumors.

Peter Choyke, M.D.

Molecular Imaging: PET Imaging - Clinical Research

Description Point of Contact Link

The Positron Emission Tomography Department has extensive resources for PET imaging: two GE Advance whole body PET scanners, a High Resolution Research Tomograph (HRRT) for brain imaging, and a Siemens mCT PET/CT scanner. The department performs PET imaging as part of numerous intramural clinical research protocols using a wide variety of radiopharmaceuticals, primarily for neurologic, psychiatric and oncologic applications.

Peter Herscovitch, M.D.

PET Radiotracer/Ligand Synthesis: Cyclotron-Produced Radionuclides

Description Point of Contact Link

The Positron Emission Tomography Department has three medical cyclotrons to produce positron-emitting and other radionuclides, two GE PETtrace cyclotrons and a CS-30 cyclotron. These produce positron-emitting radionuclides commonly used for PET imaging, including oxygen-15, nitrogen-13, carbon-11 and fluorine-18. In addition, our CS-30 cyclotron is able to produce several non-standard PET radionuclides, including copper-64 (half-life 12.7 h), yttrium-86 (14.7 h), bromine-76 (16.2 h), zirconium-89 (78 h), and iodine-124 (100 h).

Peter Herscovitch, M.D.

Radiology and Imaging Sciences Department

Description Point of Contact Link

Magnetic Resonance Imaging (MRI): 3 Tesla, 1.5 Tesla. Human whole body MRI scanners (Siemens, Philips) for head, body and musculoskeletal MRI studies.

Cardiac/Coronary CT Angiography: 128, 256 and 320 slice multi-detector scanners for high resolution studies of myocardial function, coronary calcium and coronary angiography.

Whole-Body MRI/PET Multi-Modality Imaging: Siemens Biography whole body integrated MRI-PET scanner for cancer, cardiovascular and inflammatory disease evaluation.

Research PACS/Clinical PACS Services: Image storage systems for standard clinical interpretation of radiological images. 80 Tb research archiving with web based distribution and segmentation data for individual research programs. All systems are fully redundant with off-site backup web, and handheld device access.

Clinical Image Processing Services: Image processing services designed for derivation of quantitative data extraction from medical images, including tissue segmentation, tumor identification and measurement.

Cardiac Magnetic Resonance (CMR): Full evaluation capabilities for quantification of myocardial structure, function and composition as well as atherosclerotic plaque quantification and change over time.

Computed Tomography: 64, 128, 320 slice scanners: full range of body, musculoskeletal and neurologic applications of CT, CT angiography, virtual colonoscopy and other advanced applications , with low radiation dose capability.

Wireless x-ray/fluoroscopy: Full digital capability for all x-ray capabilities including wireless ICU capability for intensive care unit and portable x-ray resulting in rapid distribution of images.

Ultrasound (portable, dedicated): whole body assessment using both portable and dedicated radiology department units for applications such as abdominal and vascular evaluation.

DEXA: bone densitometry services.

SPECT: single photon emission capability with computed tomography co-localization.

Bone Scan: Technetium planar as well as SPECT bone scan capability as well as advanced F18 PET bone scan.

Elizabeth C. Jones, M.D., M.P.H., M.B.A

Age-Related Macular Degeneration and Cataract

Description Point of Contact Link

The NEI Age-Related Eye Disease Study 2 (AREDS2) is a study of age-related macular degeneration and cataract. NEI is testing oral supplements of fish oil and vitamins (lutein/zeaxanthin) which are found in green leafy vegetables for the treatment of these two eye conditions. Additionally, NEI is testing whether these supplements have any effect on heart disease and cognitive function.

Emily Chew, M.D.


Functional and Applied Biomechanics for Motion Analysis

Description Point of Contact Link

The Functional and Applied Biomechanics Section in the Rehabilitation Medicine Department can perform state-of the science 3D motion analysis using a novel overground harness track for a diverse patient population, including those who have limited standing or walking ability. The section also has the ability to assess gait performance in simulated real world environments using a custom virtual reality system, force instrumented treadmill and a specialized control algorithm that drives the treadmill belt using 3D motion recorded from the subject using our camera system. Additionally, the section can assess balance disorders in multiple rehabilitation populations.

Diane L. Damiano, Ph.D., PT

Human Energy & Body Weight Regulation Core

Description Point of Contact Link

The Human Energy & Body Weight Regulation Core, established in 2007, is a part of the NIH Intramural Metabolic Clinical Research Unit (MCRU) and exists to develop and provide specialized facilities for comprehensive and collaborative research on factors associated with obesity and metabolic disorders. The MRC conducts intramural research protocols, establishes productive scientific collaborations, provides expertise and support for the development of clinical protocols, and trains the next generation of metabolism and obesity researchers. Specific Resources and Equipment include: metabolic kitchen, food vending machines, experimental dining room, three rapid response room calorimeters, physical activity monitoring, exercise lab, body composition unit, skilled nursing to perform dynamic endocrine testing and isotope studies.

Kong Chen, PhD

Monica Skarulis, MD

Marc L. Reitman, M.D., Ph.D.

Rehabilitation Medicine

Description Point of Contact Link

The Rehabilitation Medicine Department has the capability to perform extensive phenotyping of children and adults for research purposes. The department has extensive experience in the analysis of complex and rare diseases as well as acquired disorders from trauma.

Leighton Chan, M.D., MPH

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This page was last updated: 04/16/2019