The JCV or BKV polyomaviruses are widely distributed in the human population. Measurement of antibodies directed to these viral pathogens is done using the major virion capsid protein, VP1, as the antigen cloned and expressed in Baculovirus vectors. Enzyme linked immunoabsorbent technology, ELISA, assays are used to titrate serum/plasma samples in 4 fold dilutions in plates treated with viral protein. Antibody levels are determined as the reciprocal of the highest dilution that gives an optical density of a chromophore above preset controls run on the same plate.

Gene Major, Ph.D.
majorg@ninds.nih.gov
301.496.1635

http://neuroscience.nih.gov/Lab.asp?Org_ID=530